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Year : 2022  |  Volume : 15  |  Issue : 7  |  Page : 95-96  

ELISA IgG versus CMIA IgG for detection of SARS-CoV-2-specific antibodies in COVID-19 patients


1 TWS Center, Bangkok, Thailand
2 Dr. D.Y. Patil University, Pune, Maharashtra, India; Joseph Ayobalola University, Ikeji, Arakeji, Nigeria

Date of Submission28-Mar-2021
Date of Decision15-Sep-2021
Date of Acceptance18-Sep-2021
Date of Web Publication28-Jan-2022

Correspondence Address:
Pathum Sookaromdee
TWS Center, Bangkok
Thailand
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/mjdrdypu.mjdrdypu_226_21

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  Abstract 


COVID-19 is an important coronavirus infection. The detection of SARS-CoV-2-specific antibodies is important in laboratory medicine. Several serological tests are available. Some serological tests such as ELISA IgG versus CMIA IgG are used as standard references for rapid diagnostic immunoassay. Here, the authors reappraised on the diagnostic property of ELISA IgG versus CMIA IgG for the detection of SARS-CoV-2-specific antibodies in COVID-19 patients. According to the analysis, CMIA IgG has a higher sensitivity and ELISA IgG has a higher specificity. Therefore, CMIA IgG is more appropriate for screening purpose.

Keywords: Antibody, CMIA IgG, diagnosis, ELISA, SARS-CoV-2


How to cite this article:
Sookaromdee P, Wiwanitkit V. ELISA IgG versus CMIA IgG for detection of SARS-CoV-2-specific antibodies in COVID-19 patients. Med J DY Patil Vidyapeeth 2022;15, Suppl S1:95-6

How to cite this URL:
Sookaromdee P, Wiwanitkit V. ELISA IgG versus CMIA IgG for detection of SARS-CoV-2-specific antibodies in COVID-19 patients. Med J DY Patil Vidyapeeth [serial online] 2022 [cited 2022 Sep 27];15, Suppl S1:95-6. Available from: https://www.mjdrdypv.org/text.asp?2022/15/7/95/336817




  Introduction Top


COVID-19 is an important coronavirus infection that has been occurred since 2019. At present, more than 120 million COVID-19 cases are accumulated reported worldwide. For the management of COVID-19, the diagnosis of disease is very important. The detection of SARS-CoV-2-specific antibodies is important in laboratory medicine.[1] Several serological tests are available.[2],[3] Some serological tests such as ELISA IgG versus CMIA IgG are used as standard references for rapid diagnostic immunoassay.[4] Here, the authors reappraised on the diagnostic property of ELISA IgG versus CMIA IgG for the detection of SARS-CoV-2-specific antibodies in COVID-19 patients. According to the analysis, CMIA IgG has a higher sensitivity and ELISA IgG has a higher specificity. Therefore, CMIA IgG is more appropriate for screening purpose.


  Materials and Methods Top


The authors studied on the diagnostic test of ELISA IgG (EUROIMMUN, Lubeck, Germany) versus CMIA IgG (Abbott Ireland Diagnostics Division, Sligo, Ireland) for detection of SARS-CoV-2-specific antibodies in COVID-19 patients. The appraisal is done on cross-sectional data from 245 patients with SARS-CoV-2 infection confirmation by real-time reverse transcriptase-polymerase chain reaction. A direct comparison of diagnostic sensitivity and specificity is done. This work is a reappraisal analysis on available laboratory data, and there is no collection of personal information of the patients. The study protocol was approved by the local Research Ethics.


  Results Top


Comparing between ELISA IgG and CMIA IgG [Table 1], ELISA IgG has a higher sensitivity (0.9%) but lower specificity (10.6%) than CMIA IgG.
Table 1: Diagnostic property comparing between two tests

Click here to view



  Discussion Top


Serological diagnosis of COVID-19 is widely used at present. Many new diagnostic tools are available. The rapid serodiagnosis point of care testing becomes a useful tool. In our setting, the rapid test is usually referred to the standard diagnosis by ELISA IgG or CMIA.[4] Of interest, although both ELISA IgG and CMIA IgG tests are mentioned as gold standard,[2],[3],[4] there are differences in diagnostic properties of both tests. Here, the authors perform a reappraisal analysis for comparing between ELISA IgG and CMIA IgG for the detection of SARS-CoV-2-specific antibodies in COVID-19 patients.

According to the analysis, CMIA IgG has a higher sensitivity and ELISA IgG has a higher specificity. Therefore, CMIA IgG is more appropriate for screening purpose. This observation is concordant with a recent report that ELISA IgG test is superior to other immunological tests for screening for COVID-19.[5]

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.



 
  References Top

1.
Ciotti M, Benedetti F, Zella D, Angeletti S, Ciccozzi M, Bernardini S. SARS-CoV-2 infection and the COVID-19 pandemic emergency: The importance of diagnostic methods. Chemotherapy 2021;66:17-23.  Back to cited text no. 1
    
2.
Loeffelholz MJ, Tang YW. Laboratory diagnosis of emerging human coronavirus infections-The state of the art. Emerg Microbes Infect 2020;9:747-56.  Back to cited text no. 2
    
3.
Zainol Rashid Z, Othman SN, Abdul Samat MN, Ali UK, Wong KK. Diagnostic performance of COVID-19 serology assays. Malays J Pathol 2020;42:13-21.  Back to cited text no. 3
    
4.
Chansaenroj J, Yorsaeng R, Posuwan N, Puenpa J, Sudhinaraset N, Chirathaworn C, et al. Detection of SARS-CoV-2-specific antibodies via rapid diagnostic immunoassays in COVID-19 patients. Virol J 2021;18:52.  Back to cited text no. 4
    
5.
Jahrsdörfer B, Kroschel J, Ludwig C, Corman VM, Schwarz T, Körper S, et al. Independent side-by-side validation and comparison of 4 serological platforms for SARS-CoV-2 Antibody Testing. J Infect Dis 2021;223:796-801.  Back to cited text no. 5
    



 
 
    Tables

  [Table 1]



 

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