Assessment of resistance to second line anti-tubercular drugs by line probe assay at a tertiary care hospital Thosani P, Agarwal A, Karade S, Sen S, S. Katoch C D, Jindamwar P, S. Shergill S P, - J Indian Prosthodont Soc

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Assessment of resistance to second line anti-tubercular drugs by line probe assay at a tertiary care hospital Pratik Thosani¹, Ashwini Agarwal², Santosh Karade¹, Sourav Sen³, CD S. Katoch⁴, Prashant Jindamwar⁵, SP S. Shergill¹ ¹ Department of Microbiology, Armed Forces Medical College, Lavale, India ² Department of Microbiology, AIIMS, Jodhpur, Rajasthan, India ³ Department of Microbiology, Symbiosis Medical College for Women, Lavale, India ⁴ Department of Pulmonary Medicine, Army Institute of Cardio-thoracis Sciences, Pune, India ⁵ Department of Microbiology, Army Institute of Cardio-thoracis Sciences, Pune, India Correspondence Address: Santosh Karade, Department of Microbiology, Armed Forces Medical College, Pune - 411 040, Maharashtra India Source of Support: None, Conflict of Interest: None DOI: 10.4103/mjdrdypu.mjdrdypu_392_21

Context: Emergence and spread of extensively drug resistance (XDR) tuberculosis (TB) is a challenge in resource-limited settings. Although India accounts for one-fourth burden of multi-drug resistant cases of TB, the prevalence of XDR TB varies widely. Timely detection of resistance to anti-tubercular drugs is important for optimal case management. Aims: Characterization of resistance mutations to second-line anti-tubercular therapy (ATT) using line probe assay (LPA). Materials and Methods: In this cross-sectional, conducted at a Tertiary Care Teaching Hospital in Western Maharashtra, we assessed resistance to second-line antitubercular drugs in 100 consecutive sputum specimens from patients failing first-line ATT as per national guidelines by LPA. Results: Of the 100 specimens studied, a total of 94 assay results were valid. Of these, no resistance was seen in 52 specimens. Resistance to fluoroquinolones (FLQ) was detected in 30% of specimen with a predominance of gyrA gene mutations. A total of 12% isolates were resistant to aminoglycosides with mutations observed in rrs gene. Conclusions: The study demonstrates the utility of LPA as a tool to support the diagnosis of drug-resistant TB in clinical setting.

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    -  Thosani P
    -  Agarwal A
    -  Karade S
    -  Sen S
    -  S. Katoch C D
    -  Jindamwar P
    -  S. Shergill S P

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