Comparative scores of ABO and Rh hemagglutination reactions
Prashant Devidas Khuje1, Mahesh S Karandikar1, Bharti U Sable2
1 Department of Physiology, Dr. D. Y. Patil Medical College, Hospital and Research Centre, Pimpri, Pune, India
2 Sable Pathology Laboratory, Warora, Chandrapur, Maharashtra, India
Prashant Devidas Khuje,
Department of Physiology, DR.D.Y.P.M.C, Pimpri, Pune, 411018, F-104, Wing-H, Eden-II, Mahindra Royale, CTS 6020, Pimpri, Pune - 411 018, Maharashtra
Source of Support: None, Conflict of Interest: None
Background: Blood group determination is done by the slide test and tube test. Grading/scoring of hemagglutination reactions is performed through the naked eye and microscopy to detect spurious (false positive and negative) reactions. Objectives: In our observational study, comparative scoring of ABO and Rh hem-agglutination reactions was done based on degree of hemolysis and agglutination through the macroscopic and microscopic technique. Materials and Methods: The determination of blood group of 209 participants was done by the porcelain slide method using commercially prepared antisera (manufactured by TULIP DIAGNOSTICS (P) LTD.) containing anti-A, anti-B, and anti-D monoclonal antibodies followed by scoring/grading based on degree of hemolysis and red blood cell clumping. The data were statistically analyzed using the Chi-square test and Z-test of proportion. Results: ABO hemagglutination reactions showed significantly higher score ([A-Macro Z = 7.153, Micro Z = 4.65] [B-Macro Z = 11.17, Micro Z = 21]) (Z >2.5, P < 0.001) than Rh hemagglutination reactions. B hemagglutination reactions showed insignificantly higher score (B-Macro Z = 1.29, Micro Z = 1.816) (Z <1.95, P < 0.05) than A hemagglutination reaction. Conclusion: ABO hemagglutination reactions showed significantly higher score than Rh hemagglutination reactions as ABO system agglutinin are pentavalent (immunoglobulin M) binding 10 agglutinogens while that of Rh system are bivalent binding 4 agglutinogens. B hemagglutination reactions showed insignificantly higher score than A hem-agglutination reaction which required further evaluation.